WebOct 11, 2024 · In the RagC-S75N GDP structure, OG1 of the Thr 90 side chain is close to the O2′ of the bound nucleotide (3.7 Å), so it is likely that the larger T90N substitution leads to … WebPlasmid pRK5 Flag-RagC from Dr. David Sabatini's lab contains the insert RagC and is published in Mol Cell. 2024 Oct 17. pii: S1097-2765(17)30704-9. doi: 10.1016/j.molcel.2024.09.026. This plasmid is available through Addgene. ... pRK5 Flag-RagC(S75N) pRK5 HA-RagA pRK5 HA-RagA(T21N) Sign Up for Our Newsletter. Receive …
TSC2 regulates lysosome biogenesis via a non-canonical RAGC …
WebOpen gel images with ImageLab software (Bio-Rad, Inc.) and quantify band intensities using one of the following methods: a. Gel lane method: Under the “Lane and Bands” submenu, use the “Lane Finder” tool or manually set the number of lanes and manually adjust the lanes to fit the gel image. WebJan 25, 2024 · The phosphorylation of TFEB by the mechanistic target of rapamycin complex 1 (mTORC1)2–5 is unique in its mTORC1 substrate recruitment mechanism, which is strictly dependent on the amino... colin fairbrother
Selfridge Air National Guard Base (ANGB) Directory
WebFeb 15, 2024 · Rick Wershe, Blake’s Hard Cider involved in project. After sitting vacant for years, the old Gibraltar Trade Center site along I-94 in Mount Clemens is set to get a total … WebOct 27, 2016 · The activation of mTORC1 by leucine has been shown to be mediated by the small GTPases RagA/B and RagC/D (Kim et al., 2008), and overexpression of constitutively active RagA Q66L /RagC S75N can bypass leucine to activate mTORC1. We found that activation of mTORC1 by either RagA Q66L or RagC S75N remained sensitive to CMDZ . … WebJul 6, 2008 · Surprisingly, expression of dominant-negative RagCS75N, but not constitutively active RagCQ120L, reproducibly increased S6K phosphorylation ( Fig. 3a ). Although less marked, this effect on S6K... coline women\u0027s sleeveless top