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Hoechst 33342 apoptosis

Nettet16. sep. 2024 · A staining dye solution was prepared by adding 1 µL of calcein AM (4 mM stock from Invitrogen), 1 µL Hoechst 33342, and 10 µL CellEvent ® Caspase-3/7 into 8 mL of staining buffer. The staining dye solution was dispensed at 1 µL per microwell in the microwell chips which was then combined with the micropillar chip containing the cells … Nettet1. sep. 2016 · First, Hoechst 33,342 stains all cell nuclei in cultures, while GC150-P2a-mApple-NLS or mApple-NLS alone can be specifically targeted to express in cell types of interest such as with a...

Determining the contents and cell origins of apoptotic bodies by …

Nettet1. sep. 1994 · Abstract A flow cytometric method to detect apoptotic cells is described. This method is based on the detection of differences in chromatin condensation with Hoechst 33342 as a probe and the detection of dead cells with propidium iodide as a probe for membrane damage. NettetInvitrogen Hoechst 33342 nucleic acid stain is a popular cell-permeant nuclear counterstain that emits blue fluorescence when bound to dsDNA. This dye is often used … peterborough timbermen twitter https://whatistoomuch.com

Use of Hoechst 33342 staining to detect apoptotic changes in …

NettetHoechst 33342 and propidium iodide are frequently used together for simultaneous flow cytometric and fluorescence imaging analysis of the stages of apoptosis and cell-cycle … NettetHoechst 33342, a vital DNA-specific fluo-rochrome, was added for determinations of DNA content and subsequent cell cycle analysis (Fig. 1). There is a decrease in the Hoechst 33342 fluorescence in the apop-totic population compared with the nonapoptotic popu-lation. The incorporation of the intracellular tandem GFP-FRET Nettet18. feb. 2024 · For this experiment, the mechanism for which cell death ensues can be elucidated by the use of two different stains: Hoechst 33342 and propidium iodide. In the case of apoptotic cells with intact membranes, Hoechst 33342 is able to permeate through the membrane to stain the DNA of cells. peterborough timbermen lacrosse

Hoechst 33342 Solution (20 mM) - Thermo Fisher Scientific

Category:A High Throughput Apoptosis Assay using 3D Cultured Cells

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Hoechst 33342 apoptosis

A High Throughput Apoptosis Assay using 3D Cultured Cells

NettetThe adenosine-induced apoptosis was determined by Hoechst 33342 staining and flow cytometry. The effect of adenosine on the intracellular reactive oxygen species (ROS) and mitochondrial membrane potential (MMP) was assessed according to the DCFH-DA and JC-1 methods, respectively. Nettet您好！欢迎来到炼石商城 请登录 注册 我的订单; 我的炼石

Hoechst 33342 apoptosis

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Nettet18. jun. 2016 · Furthermore, compound 1 dose-dependently induced A2780 cells apoptosis as evidenced by Hoechst 33342 staining, Annexin V positive cells, the up-regulated cleaved-PARP and the enhanced Bax/Bcl-2 ratio. What’s more, compound 1 also promoted the expression of the tumor suppressor p53 protein. NettetHoechst 33258 nucleic acid stain is a popular nuclear counterstain that emits blue fluorescence when bound to dsDNA. Its uses are similar to Hoechst 33342 for counterstaining, apoptosis and cell cycle studies, but Hoechst 33258 is reportedly less cell-permeant. It also has been used to detect DNA in solution between 250ng/ml to …

Nettet28. feb. 2024 · The signals of early and late apoptotic cells were located in the lower and upper right quadrant of the resulting dot plot, respectively. In Hoechst 33342 staining, the cells were washed twice with PBS and stained with Hoechst 33342 (Beyotime Biotech) at 37°C for 20 minutes in the dark. Nettet25. des. 2008 · Hoechst 33342, but not Hoechst 33258, induced apoptosis in the HL-60 cells in a time- and dose-dependent manner. Endogenous nuclear topoisomerase I activity in HL-60 cells was inhibited by ...

NettetIn this study we demonstrate that the common live-cell DNA stain Hoechst 33342 can cause apoptosis under CTLM conditions. Although toxicity is evident at long times in the absence of imaging at high dye concentrations, phototoxicity from repeated excitation of the dye in the imaging process is dominant.

Nettet31. okt. 2024 · First, we monitored the distribution of nuclear contents by staining human Jurkat T cells with the cell-permeable DNA binding dye Hoechst 33342 prior to induction of apoptosis. Next, cells...

Nettet1. okt. 1999 · In BC3H-1 myocytes, Hoechst 33342–induced apoptosis is independent of de novo RNA and protein synthesis and p53 expression.29 However, lactate oxidation … peterborough tile storeshttp://www.pathology.washington.edu/research/labs/rabinovitch/flowroom/protocols.php?p=3 stargirl tpw twitterNettet15. des. 2024 · To evaluate the anti-apoptosis property of extracellular vesicles from hP-MSCs, the Hoechst 33342 (for 10 min) is used to distinguish the apoptotic cells in the three different groups. Hoechst 33342 trihydrochloride purchased from MCE. stargirl table of contentsNettet27. mar. 2015 · The nuclear morphology of the cells was evaluated using the cell-permeable, DNA-specific fluorescent dye, Hoechst 33342. Cells with homogeneously stained nuclei were considered viable, whereas the presence of chromatin condensation and/or fragmentation was indicative of apoptosis. The C2C12 myotubes were seeded … star girls empowermentNettetHoechst 33342 is a specific fluorescent DNA stain that binds within the minor groove of double-stranded AT-rich regions. This stain can be used on both live and fixed cells (1). Portugal, J. and Waring, M.J. (1988) … star girl the bookNettetThis protocol determines the absolute number of live (Hoechst 33342 positive/PI negative, early apoptotic (Hoechst 33342 positive/PI negative, SYTO 11 low), dead (PI positive) cells and debris signals. Thus, one can follow cells … star girl tommy richman lyricsNettet15. sep. 2024 · Cell migration was detected using wound healing assay. Apoptosis, mitochondrial membrane potential (Δψ m) and reactive oxygen species (ROS) was … stargirl\u0027s award winning speech