2024 Hbad-mcherry-egfp-lc3

Hbad-mcherry-egfp-lc3

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August undefined, 2024

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WebWe detail here a protocol using tandem-tagged mCherry-EGFP-LC3 (C-G-LC3) to quantify autophagic flux in single cells by ratiometric flow cytometry and to isolate subpopulations … WebTransgenic GFP-LC3 mice are a valuable tool to investigate autophagy ; however, comparison of homeostatic and disease responses between GFP-LC3 and wild-type (WT) mice has not been done. We examined the effects of GFP-LC3 expression on autophagy, acinar cell function, and experimental pancreatitis. swtor shadow spawn quest

Following autophagy step by step BMC Biology Full Text

Web南模生物. Language: 中文 . English; 日本語; 한국어; 账户设置; 退出 WebApr 7, 2024 · LC3B-eGFP-mCherry assay by confocal and flow cytometry. LC3B-eGFP-mCherry L6 cells were plated on 6-well plates and cultured to reach 60% confluency for confocal and 80% confluency for flow cytometry. WebThe mouse was infected with mCherry-EGFP-LC3 adeno-associated virus (100 μL) via the tail vein four weeks before sacrifice. The liver was removed carefully and trimmed immediately after the mouse was sacrificed. Then, the tissue was embedded in optimal cutting temperature (OCT) compound (Sakura, Torrance, USA), serial sections (6 μm) … textpad hex editor

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WebMay 31, 2012 · Tandem fluorescent-tagged LC3 (mRFP-EGFP-LC3) is a convenient assay for monitoring autophagic flux based on different pH stability of EGFP and mRFP fluorescent proteins. However, it has been reported that there is still weak fluorescence of EGFP in acidic environments (pH between 4 and 5) or acidic lysosomes. A detailed list of materials is provided in Table 6. Important: All hits in screening approaches have to be verified using orthogonal methods to assess autophagy (for a comprehensive … See more A detailed list of materials is provided in Table 5. 1. 2.1. Grow cells in either F-bottom (adherent cell types) or V-bottom (suspension cell types) 96-well plates. Seeding of 50,000 cells per well for adherent cells and … See more For the construction of the cell lines use low passage numbers. For accurate autophagy measurements, make sure that the cells never overgrew, even during the construction of the … See more swtor shady figureWebAug 24, 2024 · First, a previously cloned pLenti-X1-pEF-mCherry-eGFP-LC3 construct (by LR clonase reaction) was cut using BamHI and XbaI to liberate the insert. All genes of interest were then Gibson assembled using Gibson Assembly Master Mix (E2611; NEB) together with the desired epitope or fluorescence tag into the pLenti-X1-DEST vector … swtor shadow spawn meditate

"WebGenome-wide association studies (GWAS) have identified hundreds of genetic risk regions for kidney dysfunction [estimated glomerular filtration rate (eGFR)]; however, the … " - Hbad-mcherry-egfp-lc3

Hbad-mcherry-egfp-lc3

mcherry-GFP-Lc3 reporter cells enable flow cytometric quantification

WebTo get more information about a location, hover your mouse over the location. WebNov 19, 2024 · HBAD-mcherry-EGFP-LC3 adenovirus (MOI = 50) was used to infect rat articular chondrocytes for 24 h according to the manufacturer’s instructions (Hanbio, Shanghai, China).

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WebpCMV-GFP-LC3B是碧云天自行研发的在哺乳动物细胞中表达GFP标签的人源LC3B 融合蛋白的质粒。该质粒含有CMV启动子，为卡那霉素抗性，转染细胞后能够在靶细胞中高效表达绿色荧光蛋白GFP标签的LC3B的融合蛋白，呈现明亮的绿色荧光，可以用于细胞自噬(autophagy)的研究。 WebTandem fluorescent-tagged LC3 (mRFP-EGFP-LC3) is a convenient assay for monitoring autophagic flux based on different pH stability of EGFP and mRFP fluorescent proteins. …

WebLC3 is a commonly used marker for measuring autophagic flux. mRFP-GFP-LC3 functions as an autophagy bionsensor via the same mechanism as Rosella: GFP is quenched in … WebThe reporter we prefer is mCherry-EGFP-LC3 expressed either off of a CMV promoter via a plasmid or off of the viral LTR in the pBABE retrovirus. Both plasmid-and retrovirus-mediated expression ...

WebC3003-100μl. Lenti-EGFP-LC3B (10^9TU/ml) 100μl. 2992.00元. Lenti-EGFP-LC3B，即Lentivirus expressing EGFP-LC3B fusion protein，是一种可以表达EGFP-LC3B融合蛋白的慢病毒，可以用于感染细胞或组织后进行细胞自噬 (autophagy)检测。. 本产品带有嘌呤霉素 (puromycin)抗性，感染细胞后可以筛选 ... WebPlasmid pBABE-puro mCherry-EGFP-LC3B from Dr. Jayanta Debnath's lab contains the insert microtubule-associated protein 1 light chain 3 beta and is published in EMBO Rep. …

WebMar 9, 2024 · Cell autophagic flux was analyzed by measuring tandem fluorescence-tagged LC3 reporter (mCherry-EGFP-LC3). Levels of p53, phosphorylated p53 ( p -p53), p21, BECN1, p62, p -mTOR and p -p70S6K were measured by western blot.

WebmCherry, tdTomato, Red Fluorescent Protein (dsRed) Cross Reactivity. This antibody does not cross-react with green fluorescent protein. Immunogen. Recombinant mCherry … swtor shadow syndicate vendorWebJun 2, 2011 · LC3 is a mammalian homolog of the yeast ATG8 protein, a ubiquitin-like protein that becomes lipidated and tightly associated with the autophagosomal membranes. LC3 is also important in the process of selective autophagy, whereby intracellular components such as protein aggregates, organelles and pathogens are removed [ 3 ]. swtor setup.exeWebCurrent Weather. 5:11 AM. 47° F. RealFeel® 48°. Air Quality Excellent. Wind NE 2 mph. Wind Gusts 5 mph. Clear More Details. swtor shadow kinetic combatWeb(2) tfLC3 mice express RFP-EGFP-LC3 or mCherry-EGFP-LC3 for measuring autophagy ﬂux. The num-bers of GFP CRFP puncta (yellow) and RFP puncta (red) are counted. Yellow puncta represent phagophores and autophagosomes, and red puncta represent autolyso-somes. (3) GFP-LC3-RFP-LC3DG mice express GFP-LC3-RFP-LC3DG for … swtor shadow tanking guideWebTHOR Directory Facility Standards; Revised September 23, 2013; Page 6 of 18 II. Structured Housing Standards Structured Housing is approved for inmates who meet prison-release eligibility swtor shared storageWebMar 23, 2024 · Lifespan. The average Cherry Barb lifespan is between 5 and 7 years. This lifespan is assuming a good level of care when it comes to water conditions, habitat … swtor shadow of revan the enemy withinWebDec 27, 2024 · LC3（light chain 3）简称于MAP1LC3 (microtubule-associated proteins light chain 3)，是目前公认的自噬标记物。哺乳动物中的LC3与酵母中的自噬相关蛋 … swtor shae vizla customization