WebBy combining chromatin immunoprecipitation (ChIP) assays with sequencing, ChIP sequencing (ChIP-Seq) is a powerful method for identifying genome-wide DNA binding sites for transcription factors and other proteins. Following ChIP protocols, DNA-bound protein is immunoprecipitated using a specific antibody. The bound DNA is then coprecipitated ... WebMonoclonal ANTI-FLAG ® M2 antibody produced in mouse. clone M2, purified immunoglobulin (Purified IgG1 subclass), buffered aqueous solution (10 mM sodium …
DYKDDDDK Tag (9A3) Mouse mAb (Binds to same epitope as Sigma
WebImmunoprecipitation ( IP) is the technique of precipitating a protein antigen out of solution using an antibody that specifically binds to that particular protein. This process can be used to isolate and concentrate a particular protein from a sample containing many thousands of different proteins. Immunoprecipitation requires that the antibody ... WebApplication. Anti-FLAG Tag antibody produced in rabbit has been used in: western blotting. co-immunoprecipitation. immunofluorescence studies. Applications in which this … reading word search puzzles printable
SMAD3 promotes expression and activity of the androgen …
WebLane 1 is 10% input, lane 2 is HA-Tag (C29F4) Rabbit mAb, and lane 3 is Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900. Western blot was performed using HA-Tag (C29F4) Rabbit mAb. A conformation specific secondary antibody was used to avoid reactivity with IgG. Immunohistochemical analysis of paraffin-embedded COS cells, untransfected (right ... WebAug 2, 2024 · ChIP-seq was performed using an anti-FLAG antibody for dCas9 binding specificity and a histone 3 lysine 9 trimethylation (H3K9me3) antibody to detect histone methylation as a result of dCas9 KRAB ... WebApr 4, 2024 · Due to the lack of a specific antibody against CTCF-s, we introduced a FLAG-tagged CTCF and CTCF-s into HeLa-S3 cells, ... suggesting good data quality for FLAG ChIP-seq, although the overall ... reading words for 2nd graders